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Objective:To investigate the experience of rehabilitation exercise for the prevention of upper limb lymphedema in patients with breast cancer rehabilitation, to provide feasible and operative activities and exercise rehabilitation information for clinical health care personnel to guide exercise rehabilitation programs for breast cancer patients after discharge. Methods:From July, 2017 to June, 2018, individualized face-to-face in-depth interviews were performed in breast cancer rehabilitation patients who had been performing upper limb lymphedema exercise for more than three times a week. The content analysis was used to analyze the data. Results:Totally, 13 candidates for breast cancer rehabilitation were selected to provide experience, and their experience of breast cancer rehabilitation in preventing upper limb lymphedema were extracted, including the type, name, time, frequency and intensity of exercise, precautions, and detailed operational experience in sports rehabilitation. Conclusion:The exercise rehabilitation experience of breast cancer rehabilitation patients in preventing upper limb lymphedema have been drawn, which may enrich and refine the current behavior guidelines for breast cancer rehabilitation patients to prevent upper limb lymphedema, and help to deepen the prevention of breast cancer rehabilitation. The understanding of the actual situation of upper limb lymphedema provides a reference for preventive interventions for the development of lymphedema in medical staff.
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Objective To test the reliability and validity of Chinese version of Illness Cognition Questionnaire(ICQ)in women with breast cancer. Methods From October,2016 to May,2017,297 women with breast cancer were investigated with the Chinese version of the ICQ and Brief Illness Perception Questionnaire(B-IPQ).The reliability and validity of the Chinese version of ICQ were tested. Results The Cronbach's α of three subscales of ICQ(helplessness,acceptance and perceive benefits)were 0.855,0.857, 0.878,and the test-retest reliability of three subscales were 0.946,0.963,0.901(P<0.001).A confirmatory factor analysis was tested,χ2/df=2.500,SRMR=0.063,CFI=0.920.The factor load capacity of each subscales was 0.37 to 0.84 and the correlation of helplessness,acceptance and perceive benefits was-0.49 to 0.81(P<0.001).The correlation coefficient of criterion-related validity of the helplessness subscale of ICP with B-IPQ was 0.727(P<0.001). Conclusion The Chinese version of ICQ has good reliability and validity,which is a valuable research tool in clinical studies of psychological cognition of patients with breast cancer.
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Objective To compare the clinical efficacy and treatment duration between two methods,tuina plus acupuncture at Qiaogong point predominantly versus conventional tuina method,in treating children with muscular torticollis.Method A hundred children with muscular torticollis were randomized into a treatment group and a control group,50 cases each.The treatment group was intervened by tuina plus acupuncture at Qiaogong point predominantly,while the control group was intervened by conventional tuina method.The clinical efficacies and treatment durations were compared between the two groups.Result The total effective rate and recovery rate were respectively 96.0％ and 80.0％ in the treatment group,versus 90.0％ and 42.0％ in the control group.There was a significant difference in comparing the recovery rate between the two groups (P＜0.05).The between-group difference in comparing the time taken for the effective and recovered cases was statistically significant (P＜0.05).Conclusion Tuina plus acupuncture predominantly at Qiaogong point can produce a more significant efficacy and it takes a shorter time in treating children with muscular torticollis compared to the conventional tuina method.
ABSTRACT
PTEN, a dual-specificity phosphatase, exerts its tumor-suppressive effects through the inhibition of cell cycle progression and cell immigration, therefore could be an important candidate for tumor-suppression. As study on prokaryotic expression of PTEN and its anti-tumor functions has not been reported, the present study aims at an efficient expression of functional PTEN in Escherichia coli and the investigation of its tumor-suppression activity. PTEN cDNA cloned in our lab previously was recombined into prokaryotic expression vector pET-44a(+) to construct pET-PTEN (pEP) and pET-Nus-PTEN (pENP). PTEN was fused with 6 x His tag in pEP, and with Nus in pENP, which could be useful for a stable and soluble expression. The recombinant vectors were transformed into both BL21 (DE3) (BL) and Rosetta-gami (DE3) pLysS (RG). The former is a normal expression host while the latter is optimized for expression of eukaryotic genes and folding of target proteins. On the induction of 0.5mmol/L IPTG, 55kD and 118kD specific protein bands were observed, corresponding to His-PTEN and Nus-PTEN fusion proteins, respectively. Western blot analysis showed the recombinant fusion proteins could react with PTEN polyclonal antibody. The recombinant HTEN was expressed both in soluble fraction and inclusion body. Higher expression levels of recombinant PTEN were obtained in BL (His-PTEN: 10.3%; NusA-PTEN: 18.7%), whereas the higher percentages of soluble recombinant proteins were observed in RG (His-PTEN: 4.7%; Nus-PTEN: 6.6%). The obtained recombinant fusion proteins were purified by affinity chromatography and were showed to be homogeneous in SDS-PAGE. In tumor-suppression experiments, His-PTEN was proved to have significant inhibition on growth of mice solid tumor with an inhibitory ratio of 58.76%, and on the proliferation of DU-145 tumor cells with an inhibitory ratio of 46.16%. The cell cycle progression of DU-145 tumor cells was also arrested from G0/G1 to S phase. His-PTEN from RG was proved to have significantly higher tumor-suppression activity than that from BL, indicating that there may be some advantages for eukaryotic genes to be expressed in the former host. This is the report of functional recombinant PTEN expressed in Escherichia coli. Purified His-PTEN was used for immunizing Kunming mice, and ascitic polyclonal antibodies raised against His-PTEN were generated using sarcoma 180 cells. At 1:2000 dilution, the antibodies could interact with PTEN by western blot. The present study has laid a foundation for application of PTEN in cancer therapy.